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rosettesep cd3 depletion reagent  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc rosettesep cd3 depletion reagent
    Antigen-specific <t>CD4</t> and CD8 memory cells show dissociated production of IL-2 and IFN-γ. Healthy subject (n = 22) PBMC ( panels A–C ) or CD4 vs. CD8 depleted PBMC ( panel D ) (300,000 cells per well) were challenged in 20 h culture with recall protein (mumps or candida) or peptide (EBV BMLF-1, EBNA3a, or EBNA3b) antigen and IFN-γ/IL-2 producing cell frequency was measured by ELISPOT method. Assays were performed in triplicate. For panels A–C each line represents a separate subject (n = 12). Panel D , CD4 vs. CD8 depletion analysis reveals immune response phenotype. Spot forming units (sfu) observed with PBMC (300,000 cells per well) were assigned as 100%. Sfu observed when plating the same number of CD4 depleted PBMC or CD8 depleted PBMC are shown as a proportion (%) of PBMC activity.
    Rosettesep Cd3 Depletion Reagent, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rosettesep cd3 depletion reagent/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    rosettesep cd3 depletion reagent - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT"

    Article Title: Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT

    Journal: Cells

    doi: 10.3390/cells1020127

    Antigen-specific CD4 and CD8 memory cells show dissociated production of IL-2 and IFN-γ. Healthy subject (n = 22) PBMC ( panels A–C ) or CD4 vs. CD8 depleted PBMC ( panel D ) (300,000 cells per well) were challenged in 20 h culture with recall protein (mumps or candida) or peptide (EBV BMLF-1, EBNA3a, or EBNA3b) antigen and IFN-γ/IL-2 producing cell frequency was measured by ELISPOT method. Assays were performed in triplicate. For panels A–C each line represents a separate subject (n = 12). Panel D , CD4 vs. CD8 depletion analysis reveals immune response phenotype. Spot forming units (sfu) observed with PBMC (300,000 cells per well) were assigned as 100%. Sfu observed when plating the same number of CD4 depleted PBMC or CD8 depleted PBMC are shown as a proportion (%) of PBMC activity.
    Figure Legend Snippet: Antigen-specific CD4 and CD8 memory cells show dissociated production of IL-2 and IFN-γ. Healthy subject (n = 22) PBMC ( panels A–C ) or CD4 vs. CD8 depleted PBMC ( panel D ) (300,000 cells per well) were challenged in 20 h culture with recall protein (mumps or candida) or peptide (EBV BMLF-1, EBNA3a, or EBNA3b) antigen and IFN-γ/IL-2 producing cell frequency was measured by ELISPOT method. Assays were performed in triplicate. For panels A–C each line represents a separate subject (n = 12). Panel D , CD4 vs. CD8 depletion analysis reveals immune response phenotype. Spot forming units (sfu) observed with PBMC (300,000 cells per well) were assigned as 100%. Sfu observed when plating the same number of CD4 depleted PBMC or CD8 depleted PBMC are shown as a proportion (%) of PBMC activity.

    Techniques Used: Enzyme-linked Immunospot, Activity Assay

    Allo-antigen-specific T cells show dissociated IL-2-IFN-γ production. Allogeneic 20 h cultures were performed as 2-way MLR with 3 × 10 5 PBMC ( panels A–C ), or as a 1-way allogeneic response using CD3/CD56 depleted PBMC stimulators (300,000 cells/well) and CD4 ( panels D–F ), or CD8 ( panels G–I ) cell responders (300,000 cells/well). IL-2 sfu ( panels A, D, G ), IFN-γ sfu ( panels B, E, H ) and proliferation cpm ( panels C, F, I ) are shown. Stimulator identification is shown on the x-axis, while responder identification is shown in the legend. Shaded regions highlight discordance between IL-2 and IFN-γ producing effector function. Stimulator or responder cell alone control cultures resulted in <5 sfu and <1,000 cpm (not shown).
    Figure Legend Snippet: Allo-antigen-specific T cells show dissociated IL-2-IFN-γ production. Allogeneic 20 h cultures were performed as 2-way MLR with 3 × 10 5 PBMC ( panels A–C ), or as a 1-way allogeneic response using CD3/CD56 depleted PBMC stimulators (300,000 cells/well) and CD4 ( panels D–F ), or CD8 ( panels G–I ) cell responders (300,000 cells/well). IL-2 sfu ( panels A, D, G ), IFN-γ sfu ( panels B, E, H ) and proliferation cpm ( panels C, F, I ) are shown. Stimulator identification is shown on the x-axis, while responder identification is shown in the legend. Shaded regions highlight discordance between IL-2 and IFN-γ producing effector function. Stimulator or responder cell alone control cultures resulted in <5 sfu and <1,000 cpm (not shown).

    Techniques Used:

    Correlations among IFN-γ, IL-2, and proliferation.
    Figure Legend Snippet: Correlations among IFN-γ, IL-2, and proliferation.

    Techniques Used:

    The allogeneic proliferative response entails a substantial non-T cell bystander component. Panel A. representative proliferative reaction by CFSE analysis. CFSE labeled allogeneic responder PBMC (300,000 cells/well) were cultured with CD3/56 depleted PBMC for 6 days. Lymphocyte gate was determined by forward and side scatter. CFSE dye dilution was analyzed on responder alone (left panels) vs. responder and stimulator (right panels) gating on CD4, CD8 and CD4-/CD8- cell fractions. Reactions for 4 separate stimulators (represented on x -axis) and responders (represented in legend) are shown in panels B–E , representing CD4 ( panel B ), CD8 ( panel C ), and CD4-/CD8- ( panel D ) proliferation by CFSE dye dilution analysis, or bulk proliferation by 3 H thymidine incorporation ( panel E ).
    Figure Legend Snippet: The allogeneic proliferative response entails a substantial non-T cell bystander component. Panel A. representative proliferative reaction by CFSE analysis. CFSE labeled allogeneic responder PBMC (300,000 cells/well) were cultured with CD3/56 depleted PBMC for 6 days. Lymphocyte gate was determined by forward and side scatter. CFSE dye dilution was analyzed on responder alone (left panels) vs. responder and stimulator (right panels) gating on CD4, CD8 and CD4-/CD8- cell fractions. Reactions for 4 separate stimulators (represented on x -axis) and responders (represented in legend) are shown in panels B–E , representing CD4 ( panel B ), CD8 ( panel C ), and CD4-/CD8- ( panel D ) proliferation by CFSE dye dilution analysis, or bulk proliferation by 3 H thymidine incorporation ( panel E ).

    Techniques Used: Labeling, Cell Culture

    During mixed lymphocyte reactions soluble factors are likely involved with proliferation. Associations between CD4 T cell and CD8 T cell ( panel A ), between CD4 T cell and CD4-/CD8- cell ( panel B ), and between CD8 T cellCD4 T cell and CD4-/CD8- cell ( panel C ) proliferation as determined by CFSE dye dilution method for reactions described in .
    Figure Legend Snippet: During mixed lymphocyte reactions soluble factors are likely involved with proliferation. Associations between CD4 T cell and CD8 T cell ( panel A ), between CD4 T cell and CD4-/CD8- cell ( panel B ), and between CD8 T cellCD4 T cell and CD4-/CD8- cell ( panel C ) proliferation as determined by CFSE dye dilution method for reactions described in .

    Techniques Used:

    Soluble antigen specifc proliferative response also entails a substantial non-T cell bystander component. PBMC from two individuals with candida specific IL-2 and IFN-γ secreting CD4 T cell populations (identified in as CD4 T cell mediated activity) were analyzed by CFSE dye dilution analysis of 6 day antigen specific proliferation reactions. Analysis of CD4, CD8, and CD4-/CD8- cell fractions proliferating in response to soluble antigen are represented for each individual.
    Figure Legend Snippet: Soluble antigen specifc proliferative response also entails a substantial non-T cell bystander component. PBMC from two individuals with candida specific IL-2 and IFN-γ secreting CD4 T cell populations (identified in as CD4 T cell mediated activity) were analyzed by CFSE dye dilution analysis of 6 day antigen specific proliferation reactions. Analysis of CD4, CD8, and CD4-/CD8- cell fractions proliferating in response to soluble antigen are represented for each individual.

    Techniques Used: Activity Assay



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    rosettesep cd3 depletion reagent  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc rosettesep cd3 depletion reagent
    Antigen-specific <t>CD4</t> and CD8 memory cells show dissociated production of IL-2 and IFN-γ. Healthy subject (n = 22) PBMC ( panels A–C ) or CD4 vs. CD8 depleted PBMC ( panel D ) (300,000 cells per well) were challenged in 20 h culture with recall protein (mumps or candida) or peptide (EBV BMLF-1, EBNA3a, or EBNA3b) antigen and IFN-γ/IL-2 producing cell frequency was measured by ELISPOT method. Assays were performed in triplicate. For panels A–C each line represents a separate subject (n = 12). Panel D , CD4 vs. CD8 depletion analysis reveals immune response phenotype. Spot forming units (sfu) observed with PBMC (300,000 cells per well) were assigned as 100%. Sfu observed when plating the same number of CD4 depleted PBMC or CD8 depleted PBMC are shown as a proportion (%) of PBMC activity.
    Rosettesep Cd3 Depletion Reagent, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rosettesep cd3 depletion reagent/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    rosettesep cd3 depletion reagent - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Antigen-specific CD4 and CD8 memory cells show dissociated production of IL-2 and IFN-γ. Healthy subject (n = 22) PBMC ( panels A–C ) or CD4 vs. CD8 depleted PBMC ( panel D ) (300,000 cells per well) were challenged in 20 h culture with recall protein (mumps or candida) or peptide (EBV BMLF-1, EBNA3a, or EBNA3b) antigen and IFN-γ/IL-2 producing cell frequency was measured by ELISPOT method. Assays were performed in triplicate. For panels A–C each line represents a separate subject (n = 12). Panel D , CD4 vs. CD8 depletion analysis reveals immune response phenotype. Spot forming units (sfu) observed with PBMC (300,000 cells per well) were assigned as 100%. Sfu observed when plating the same number of CD4 depleted PBMC or CD8 depleted PBMC are shown as a proportion (%) of PBMC activity.

    Journal: Cells

    Article Title: Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT

    doi: 10.3390/cells1020127

    Figure Lengend Snippet: Antigen-specific CD4 and CD8 memory cells show dissociated production of IL-2 and IFN-γ. Healthy subject (n = 22) PBMC ( panels A–C ) or CD4 vs. CD8 depleted PBMC ( panel D ) (300,000 cells per well) were challenged in 20 h culture with recall protein (mumps or candida) or peptide (EBV BMLF-1, EBNA3a, or EBNA3b) antigen and IFN-γ/IL-2 producing cell frequency was measured by ELISPOT method. Assays were performed in triplicate. For panels A–C each line represents a separate subject (n = 12). Panel D , CD4 vs. CD8 depletion analysis reveals immune response phenotype. Spot forming units (sfu) observed with PBMC (300,000 cells per well) were assigned as 100%. Sfu observed when plating the same number of CD4 depleted PBMC or CD8 depleted PBMC are shown as a proportion (%) of PBMC activity.

    Article Snippet: PBMC, CD3- depleted PBMC (>97% CD3- cells; RosetteSep CD3 depletion reagent; StemCell Technologies, Vancouver BC, Canada), CD3/56 depleted PBMC (>95% CD3/56- cells; RosetteSep reagent), CD4 T cells (negative selection method, RosetteSep reagent), and CD8 T cells (negative selection method using R&D systems, Inc., Minneapolis MN, USA) were freshly prepared from peripheral blood specimens.

    Techniques: Enzyme-linked Immunospot, Activity Assay

    Allo-antigen-specific T cells show dissociated IL-2-IFN-γ production. Allogeneic 20 h cultures were performed as 2-way MLR with 3 × 10 5 PBMC ( panels A–C ), or as a 1-way allogeneic response using CD3/CD56 depleted PBMC stimulators (300,000 cells/well) and CD4 ( panels D–F ), or CD8 ( panels G–I ) cell responders (300,000 cells/well). IL-2 sfu ( panels A, D, G ), IFN-γ sfu ( panels B, E, H ) and proliferation cpm ( panels C, F, I ) are shown. Stimulator identification is shown on the x-axis, while responder identification is shown in the legend. Shaded regions highlight discordance between IL-2 and IFN-γ producing effector function. Stimulator or responder cell alone control cultures resulted in <5 sfu and <1,000 cpm (not shown).

    Journal: Cells

    Article Title: Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT

    doi: 10.3390/cells1020127

    Figure Lengend Snippet: Allo-antigen-specific T cells show dissociated IL-2-IFN-γ production. Allogeneic 20 h cultures were performed as 2-way MLR with 3 × 10 5 PBMC ( panels A–C ), or as a 1-way allogeneic response using CD3/CD56 depleted PBMC stimulators (300,000 cells/well) and CD4 ( panels D–F ), or CD8 ( panels G–I ) cell responders (300,000 cells/well). IL-2 sfu ( panels A, D, G ), IFN-γ sfu ( panels B, E, H ) and proliferation cpm ( panels C, F, I ) are shown. Stimulator identification is shown on the x-axis, while responder identification is shown in the legend. Shaded regions highlight discordance between IL-2 and IFN-γ producing effector function. Stimulator or responder cell alone control cultures resulted in <5 sfu and <1,000 cpm (not shown).

    Article Snippet: PBMC, CD3- depleted PBMC (>97% CD3- cells; RosetteSep CD3 depletion reagent; StemCell Technologies, Vancouver BC, Canada), CD3/56 depleted PBMC (>95% CD3/56- cells; RosetteSep reagent), CD4 T cells (negative selection method, RosetteSep reagent), and CD8 T cells (negative selection method using R&D systems, Inc., Minneapolis MN, USA) were freshly prepared from peripheral blood specimens.

    Techniques:

    Correlations among IFN-γ, IL-2, and proliferation.

    Journal: Cells

    Article Title: Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT

    doi: 10.3390/cells1020127

    Figure Lengend Snippet: Correlations among IFN-γ, IL-2, and proliferation.

    Article Snippet: PBMC, CD3- depleted PBMC (>97% CD3- cells; RosetteSep CD3 depletion reagent; StemCell Technologies, Vancouver BC, Canada), CD3/56 depleted PBMC (>95% CD3/56- cells; RosetteSep reagent), CD4 T cells (negative selection method, RosetteSep reagent), and CD8 T cells (negative selection method using R&D systems, Inc., Minneapolis MN, USA) were freshly prepared from peripheral blood specimens.

    Techniques:

    The allogeneic proliferative response entails a substantial non-T cell bystander component. Panel A. representative proliferative reaction by CFSE analysis. CFSE labeled allogeneic responder PBMC (300,000 cells/well) were cultured with CD3/56 depleted PBMC for 6 days. Lymphocyte gate was determined by forward and side scatter. CFSE dye dilution was analyzed on responder alone (left panels) vs. responder and stimulator (right panels) gating on CD4, CD8 and CD4-/CD8- cell fractions. Reactions for 4 separate stimulators (represented on x -axis) and responders (represented in legend) are shown in panels B–E , representing CD4 ( panel B ), CD8 ( panel C ), and CD4-/CD8- ( panel D ) proliferation by CFSE dye dilution analysis, or bulk proliferation by 3 H thymidine incorporation ( panel E ).

    Journal: Cells

    Article Title: Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT

    doi: 10.3390/cells1020127

    Figure Lengend Snippet: The allogeneic proliferative response entails a substantial non-T cell bystander component. Panel A. representative proliferative reaction by CFSE analysis. CFSE labeled allogeneic responder PBMC (300,000 cells/well) were cultured with CD3/56 depleted PBMC for 6 days. Lymphocyte gate was determined by forward and side scatter. CFSE dye dilution was analyzed on responder alone (left panels) vs. responder and stimulator (right panels) gating on CD4, CD8 and CD4-/CD8- cell fractions. Reactions for 4 separate stimulators (represented on x -axis) and responders (represented in legend) are shown in panels B–E , representing CD4 ( panel B ), CD8 ( panel C ), and CD4-/CD8- ( panel D ) proliferation by CFSE dye dilution analysis, or bulk proliferation by 3 H thymidine incorporation ( panel E ).

    Article Snippet: PBMC, CD3- depleted PBMC (>97% CD3- cells; RosetteSep CD3 depletion reagent; StemCell Technologies, Vancouver BC, Canada), CD3/56 depleted PBMC (>95% CD3/56- cells; RosetteSep reagent), CD4 T cells (negative selection method, RosetteSep reagent), and CD8 T cells (negative selection method using R&D systems, Inc., Minneapolis MN, USA) were freshly prepared from peripheral blood specimens.

    Techniques: Labeling, Cell Culture

    During mixed lymphocyte reactions soluble factors are likely involved with proliferation. Associations between CD4 T cell and CD8 T cell ( panel A ), between CD4 T cell and CD4-/CD8- cell ( panel B ), and between CD8 T cellCD4 T cell and CD4-/CD8- cell ( panel C ) proliferation as determined by CFSE dye dilution method for reactions described in .

    Journal: Cells

    Article Title: Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT

    doi: 10.3390/cells1020127

    Figure Lengend Snippet: During mixed lymphocyte reactions soluble factors are likely involved with proliferation. Associations between CD4 T cell and CD8 T cell ( panel A ), between CD4 T cell and CD4-/CD8- cell ( panel B ), and between CD8 T cellCD4 T cell and CD4-/CD8- cell ( panel C ) proliferation as determined by CFSE dye dilution method for reactions described in .

    Article Snippet: PBMC, CD3- depleted PBMC (>97% CD3- cells; RosetteSep CD3 depletion reagent; StemCell Technologies, Vancouver BC, Canada), CD3/56 depleted PBMC (>95% CD3/56- cells; RosetteSep reagent), CD4 T cells (negative selection method, RosetteSep reagent), and CD8 T cells (negative selection method using R&D systems, Inc., Minneapolis MN, USA) were freshly prepared from peripheral blood specimens.

    Techniques:

    Soluble antigen specifc proliferative response also entails a substantial non-T cell bystander component. PBMC from two individuals with candida specific IL-2 and IFN-γ secreting CD4 T cell populations (identified in as CD4 T cell mediated activity) were analyzed by CFSE dye dilution analysis of 6 day antigen specific proliferation reactions. Analysis of CD4, CD8, and CD4-/CD8- cell fractions proliferating in response to soluble antigen are represented for each individual.

    Journal: Cells

    Article Title: Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT

    doi: 10.3390/cells1020127

    Figure Lengend Snippet: Soluble antigen specifc proliferative response also entails a substantial non-T cell bystander component. PBMC from two individuals with candida specific IL-2 and IFN-γ secreting CD4 T cell populations (identified in as CD4 T cell mediated activity) were analyzed by CFSE dye dilution analysis of 6 day antigen specific proliferation reactions. Analysis of CD4, CD8, and CD4-/CD8- cell fractions proliferating in response to soluble antigen are represented for each individual.

    Article Snippet: PBMC, CD3- depleted PBMC (>97% CD3- cells; RosetteSep CD3 depletion reagent; StemCell Technologies, Vancouver BC, Canada), CD3/56 depleted PBMC (>95% CD3/56- cells; RosetteSep reagent), CD4 T cells (negative selection method, RosetteSep reagent), and CD8 T cells (negative selection method using R&D systems, Inc., Minneapolis MN, USA) were freshly prepared from peripheral blood specimens.

    Techniques: Activity Assay